Sino Biological’s Protein Expression Service
Proteins are the building blocks of living cells. The specific structures of these proteins carry the genetic information encoded by genes into functional components of the cell. This process is known as gene expression, and it involves highly regulated transcription and translation processes. The information encoded by a gene is first converted into mRNA and then translated into a protein. In prokaryotic cells, transcription and translation are simultaneously coupled; the sequence of amino acids coded by a gene is transferred from mRNA to protein in one step. In eukaryotic cells, transcription and translation are separated into two steps.
What to Know About Protein Expression Services for Research
Recombinant protein production requires harnessing living cells to act as factories to construct and produce proteins based on supplied DNA templates. The cellular machinery is usually engineered with add-on signals and tags to facilitate the purification and analysis of the protein product.
Different systems can be used to produce recombinant proteins depending on the features of the target protein. For example, simpler protein structures with few post-translational modifications may be produced in bacterial or yeast systems, while complex proteins with transmembrane spans or multiple domains will likely require mammalian hosts for sufficient quality.
Sino Biological offers a comprehensive protein expression service for all types of proteins, including antibodies, enzymes, membrane proteins and cytokines. The service is supported by a comprehensive database with high throughput protein expression condition screening to ensure optimum recombinant protein yield and purity. In addition, a full range of endotoxin removal services can be offered to support downstream applications in vivo or cell-based assays.…
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